Allevi Bioprint Online: Crosslinking

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Photocrosslinking is a common method for stiffening or crosslinking hydrogels in bioprinting. One of the CORE print head’s highlights, available on the Allevi 1 and Allevi 3, is having both UV (365nm) and Blue (405nm) light crosslinking. There are three easy-to-use settings available in the Allevi software. If you do not need to use crosslinking, you can leave the settings as disabled. Follow along belong for the Allevi Bioprint Online crosslinking tips and tricks.

During Print

bioprint online crosslinking

When building tall structures from photocrosslinkable materials, you often need to crosslink many times during the print. Crosslinking after every layer (N=1) or every other layer (N=2) are common choices. When enabled, the print head will center your LED of choice over your construct and crosslink it at the set Intensity for the set Duration at the set Frequency, then resume printing.

Note: A previous version of the software turned on the crosslinking light while the layer was being extruded. To avoid uneven crosslinking of extruded material, it was revised to the above method. To keep using this previous method, enabled During Print crosslinking and leave the Duration as 0.

After Print

bioprint online crosslinking

You can increase stability by crosslinking the completed construct further. When enabled, the print head will center your LED of choice over your construct and crosslink it at the set Intensity for the set Duration.

Note: If you are printing in a multi-wellplate, the printer will perform the After Print crosslinking for each well.

Manual

bioprint online crosslinking

There may be times when you just want to turn on crosslinking. When active, the print head will turn on the LED of choice at the set Intensity wherever the print head currently is.

These settings should help you when working with bioprint online crosslinking. For any further questions, send us an email at [email protected]. Happy crosslinking!

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