Hyperelastic Bone: Troubleshooting Guide

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Hyperelastic Bone is a hydroxyapatite-based biomaterial made by Dimension Inx. This bioink can be deposited at room temperature and seeded with cells post-printing. If you followed our Hyperelastic Bone Printing protocol and encountered some problems, this troubleshooting guide will help you. 

The printed construct is very elastic and might be too soft for bone implantation.

This is absolutely normal and by design. Its soft properties allow the construct to be easily manipulated—cut, rolled, folded, or sutured. This makes it easy to print and implant, but once in the body, it calcifies and hardens, changing its material properties.

It’s difficult to draw up Hyperelastic Bone into the needle.

Hyperelastic Bone needs vigorous vortexing before use. If you find it difficult to aspirate using a needle, increase vortexing time up to 1 hour. To speed up the process we recommend using a bioink mixer

Hyperelastic Bone has dried up.

To salvage dried Hyperelastic bone, add dichloromethane dropwise and vortex vigorously in between. Continue adding until the material is at the desired consistency. To prevent it from drying, we recommend storing the material in an airtight glass container.

If your Hyperelastic Bone arrived dried up, please contact the Allevi Support Team at [email protected].

The needle clogs during Hyperelastic Bone printing.

This might be due to improper needle diameter. We recommend using needle gauge size G27 (0.2 mm inner diameter). 

I would like to clean Hyperelastic Bone residues.

If you are printing with a reusable metal syringe or using a metal or glass surface, you can follow our thermoplastic cleaning protocol.

If you have any more questions regarding Hyperelastic Bone bioprinting, you can contact the Allevi Customer Success Team at [email protected].

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