PEGSSDA allows researchers to quickly and gently dissolve HyStem® hydrogels non-enzymatically, thereby easily recovering cells or spheroids either grown on top or within the hydrogel. PEGSSDA contains two internal disulfide bonds which are rapidly reduced with small amounts of reducing agent (such as N-acetyl cysteine or glutathione), causing gel liquefaction.
PEGSSDA is a pentablock PEGDA molecule composed of four PEG molecules (MW 2000 Da) and two interdigitated disulfide bonds. Only the external PEG molecules are acrylated. The final molecular weight is approximately 8500 Da. Like PEGDA, PEGSSDA is a thiol-reactive crosslinker that covalently reacts with the thiol groups in Glycosil®, HyStem, and Gelin-S® to form viscoelastic hydrogels.
|Dimensions||2 × 2 × 2 in|
2 sterile vials containing 0.5 mL worth of PEGSSDA in lyophilized form.