Heprasil® Thiolated Heparin and Hyaluronan
Heprasil® is thiol-modified hyaluronic acid with thiol-modified heparin. Hyaluronic acid is a major constituent of native extracellular matrix (ECM). Heparin is also present in the ECM as heparan sulfate. Most cells do not attach to Heprasil-only hydrogels. Heprasil must be used in conjunction with Gelin-S® or ECM proteins such as laminin, collagen, or fibronectin for most 3-D cell culture and tissue-engineering applications.
Reconstituted Heprasil remains liquid at 15 to 37°C. Without a crosslinker, Heprasil will form a hydrogel via disulfide bond formation; however, the gelation time is over twenty-four hours. If Extralink® is used to crosslink the Heprasil, the gelation time is about 20 minutes with no low-temperature or low-pH steps. Diluting Heprasil with phosphate-buffered saline (PBS) or cell-culture medium can increase its gelation time.
|Dimensions||4 × 4 × 4 in|
5 sterile vials containing 10 mg of Heprasil® Thiolated Heparin and Hyaluronan in lyophilized form.