Current Count

Three-Dimensional Printing of Hydrogel Filters Containing Algae Cells for Copper Removal From Contaminated Water

Copper contamination of drinking water and marine areas is detrimental to human health and the environment. Physical and chemical approaches currently used for copper removal from water tend to be expensive and may introduce chemicals to the water. Using suspended algae to remove copper is a biological approach. Its cost is relatively low, and algae can be used for other

Programmable and Contractile Materials Through Cell Encapsulation in Fibrous Hydrogel Assemblies

The natural extracellular matrix (ECM)within tissues isphysically contracted and remodeled by cells, allowingthe collective shaping offunctional tissue architectures. Synthetic materials that facilitateself-assembly similar to natural ECM are neededfor cell culture, tissue engineering, and in vitro models of development and disease. To address this need,we develop fibrous hydrogel assemblies that are stabilizedwith photocrosslinkinganddisplay fiber density dependent strain responsive properties (strain-stiffening, alignment).

Gelatine-based drug-eluting bandage contact lenses: Effect of PEGDA concentration and manufacturing technique

Drug-eluting bandage contact lenses (BCLs) have been widely studied as an alternative to eye drops due to their ability to increase the drug residence time and bioavailability as well as improve patient compliance. While silicone hydrogel polymers are commonly used in drug-eluting BCLs due to their transparency, mechanical properties and high oxygen permeability, gelatine hydrogels are also clear, flexible and

Pulsed Microfluid Force-Based On-Chip Modular Fabrication for Liver Lobule-Like 3D Cellular Models

In vitro three-dimensional (3D) cellular models with native tissue-like architectures and functions have potential as alternatives to human tissues in regenerative medicine and drug discovery. However, it is difficult to replicate liver constructs that mimic in vivo microenvironments using current approaches in tissue engineering because of the vessel-embedded 3D structure and complex cell distribution of the liver. This paper reports

An immune regulatory 3D-printed alginate-pectin construct for immunoisolation of insulin producing β-cells

Different bioinks have been used to produce cell-laden alginate-based hydrogel constructs for cell replacement therapy but some of these approaches suffer from issues with print quality, long-term mechanical instability, and bioincompatibility. In this study, new alginate-based bioinks were developed to produce cell-laden grid-shaped hydrogel constructs with stable integrity and immunomodulating capacity. Integrity and printability were improved by including the co–block-polymer

In situ 3D printing of implantable energy storage devices

The increasing demand for wearable bioelectronic devices has driven tremendous research effort on the fabrication of bioelectronics in microscale. To ensure the functionality and reliability, wearable bioelectronics need to be integrated with independent and internal energy storage systems to avoid frequent charging process from external sources. The supercapacitors has been considered as an electric energy source due to benefits such

Design and 3D bioprinting of interconnected porous scaffolds for bone regeneration. An additive manufacturing approach

Scaffolds are very important element for bone regeneration issues. On this way, the purpose of this paper is the design and manufacturing of porous scaffolds fabricated by 3D printing technology from biodegradable thermoplastic polymers and calcium phosphates (in micrometric scale). So, the main aim of this research is to obtain complex porous 3D structures that present adequate mechanical properties in

3D Printing of Hydrogel Filters Containing Algae Cells for Copper Removal from Contaminated Water

Copper contamination of drinking water and marine areas is detrimental to human health and the environment. Physical and chemical approaches currently used for copper removal from water tend to be expensive and may introduce chemicals to the water. Using suspended algae to remove copper is a biological approach. Its cost is relatively low, and algae can be used for other

Tunable electroconductive decellularized extracellular matrix hydrogels for engineering human cardiac microphysiological systems

Cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs) offer tremendous potential when used to engineer human tissues for drug screening and disease modeling; however, phenotypic immaturity reduces assay reliability when translating in vitro results to clinical studies. To address this, we have developed hybrid hydrogels comprised of decellularized porcine myocardial extracellular matrix (dECM) and reduced graphene oxide (rGO) to

3D bioprinting of a cell-laden antibacterial polysaccharide hydrogel composite

Bioink with inherent antibacterial activity is of particular interest for tissue engineering application due to the growing number of bacterial infections associated with impaired wound healing or bone implants. However, the development of cell-laden bioink with potent antibacterial activity while supporting tissue regeneration proved to be challenging. Here, we introduced a cell-laden antibacterial bioink based on Methylcellulose/Alginate (MC/Alg) hydrogel for

Comparative Analysis of Chemotherapy-Induced Peripheral Neuropathy in Bioengineered Sensory Nerve Tissue Distinguishes Mechanistic Differences in Early-Stage Vincristine-, Cisplatin-, and Paclitaxel-Induced Nerve Damage

Chemotherapy-induced peripheral neuropathy (CIPN) is a well-known, potentially permanent side effect of widely used antineoplastic agents. The mechanisms of neuropathic progression are poorly understood, and the need to test efficacy of novel interventions to treat CIPN continues to grow. Bioengineered microphysiological nerve tissue (“nerve on a chip”) has been suggested as an in vitro platform for modeling the structure and

Hyaluronic Acid-Based Shape-Memory Cryogel Scaffolds for Focal Cartilage Defect Repair

Traumatic joint injuries can result in significant cartilage defects, which can greatly increase the risk of osteoarthritis development. Due to the limited self-healing capacity of avascular cartilage, tissue engineering approaches are required for filling defects and promoting cartilage regeneration. Current approaches utilize invasive surgical procedures for extraction and implantation of autologous chondrocytes; therefore, injectable biomaterials have gained interest to minimize

3D culture of HepaRG cells in GelMa and its application to bioprinting of a multicellular hepatic model

Bioprinting is an emergent technology that has already demonstrated the capacity to create complex and/or vascularized multicellular structures with defined and organized architectures, in a reproducible and high throughput way. Here, we present the implementation of a complex liver model by the development of a three-dimensional extrusion bioprinting process, including parameters for matrix polymerization of methacrylated gelatin, using two hepatic

Biofabrication of muscle fibers enhanced with plant viral nanoparticles using surface chaotic flows

Multiple human tissues exhibit fibrous nature. Therefore, the fabrication of hydrogel filaments for tissue engineering is a trending topic. Current tissue models are made of materials that often require further enhancement for appropriate cell attachment, proliferation and differentiation. Here we present a simple strategy, based on the use of surface chaotic flows amenable to mathematical modeling, to fabricate continuous, long

Bioprinting Using Algae: Effects of Extrusion Pressure and Needle Diameter on Cell Quantity in Printed Samples

Bioprinting is the fabrication of structures based on layer-by-layer deposition of biomaterials. Applications of bioprinting using plant or algae cells include the production of metabolites for use in pharmaceutical, cosmetic, and food industries. While reported studies regarding effects of extrusion pressure and needle diameter on cell viability in bioprinting have used animal cells, there previously were no reports regarding effects

Alginate-Based Bioinks for 3D Bioprinting and Fabrication of Anatomically Accurate Bone Grafts

To realize the promise of 3D bioprinting, it is imperative to develop bioinks that possess the necessary biological and rheological characteristics for printing cell-laden tissue grafts. Alginate is widely used as a bioink because its rheological properties can be modified through pre-crosslinking or the addition of thickening agents to increase printing resolution. However, modification of alginate’s physiochemical characteristics using common

Symbiotic Photosynthetic Oxygenation within 3D-Bioprinted Vascularized Tissues

In this study, we present the photosynthetic oxygen (O 2) supply to mammalian cells within a volumetric extracellular matrix-like construct, whereby a three-dimensional (3D)-bioprinted fugitive pattern encapsulating unicellular green algae, Chlamydomonas reinhardtii, served as a natural photosynthetic O 2 generator. The presence of bioprinted C. reinhardtii enhanced the viability and functionality of mammalian cells while reducing the hypoxic conditions within

Preclinical evaluation of a 3D-printed hydroxyapatite/poly(lactic-co-glycolic acid) scaffold for ridge augmentation

Background/Purpose Supracrestal ridge augmentation (SRA) is a major challenge for clinicians. This study investigated the efficacy of a 3D-printed (3DP) hydroxyapatite/poly(lactic-co-glycolic acid) (HA/PLGA) scaffold as a potential biologic for SRA. Methods Scaffolds that were 5 mm in diameter and 2.5-mm thick with a 1.2-mm diameter through-and-through central hole composed of 90% HA and 10% PLGA were printed using an extrusion-based bioprinter.

3D bioprinting of mechanically tuned bioinks derived from cardiac decellularized extracellular matrix

3D bioprinting is a powerful technique for engineering tissues used to study cell behavior and tissue properties in vitro. With the right formulation and printing parameters, bioinks can provide native biological and mechanical cues while allowing for versatile 3D structures that recapitulate tissue-level organization. Bio-based materials that support cellular adhesion, differentiation, and proliferation – including gelatin, collagen, hyaluronic acid, and

3D-Printed Veterinary Dosage Forms—A Comparative Study of Three Semi-Solid Extrusion 3D Printers

Currently, the number of approved veterinary medicines are limited, and human medications are used off-label. These approved human medications are of too high potencies for a cat or a small dog breed. Therefore, there is a dire demand for smaller doses of veterinary medicines. This study aims to investigate the use of three semi-solid extrusion 3D printers in a pharmacy