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Synthesized biocompatible and conductive ink for 3D printing of flexible electronics

Three-dimensional (3D) printing is an efficient technique for the fabrication of electronic devices. It also enables the use conductive of biomaterials in various applications, such as implants and flexible devices. Designing a new bioink is extremely challenging. For bioelectronics devices, bioink materials should be printable, flexible, conductive, harmless to cells, and sufficiently strong to maintain their shape when immersed in

Alginate Hydrogels with Embedded ZnO Nanoparticles for Wound Healing Therapy

In this in-vitro study, we designed a 3D printed composite of zinc oxide (ZnO) nanoparticles (NPs) with photocatalytic activities encapsulated within hydrogel (alginate) constructs, for antibacterial purposes applicable towards wound healing. We primarily sought to confirm the mechanical properties and cell compatibility of these ZnO NP infused scaffolds.

3D-Printed Surface-Patterned Ceramic Membrane with Enhanced Performance in Crossflow Filtration

Appropriate surface patterns on polymer membranes have been demonstrated to have a strong effect on improving the permeability and anti-fouling ability. Herein, for the first time we have developed a surface-patterned alumina ceramic membrane with gradient porous structure using a novel 3D-printing technology. The well-controllable surface-patterned alumina membrane was fabricated by use of 3D-printable ceramic ink through a layer-by-layer coating

Development of a 3D Printed Coating Shell to Control the Drug Release of Encapsulated Immediate-Release Tablets

The use of 3D printing techniques to control drug release has flourished in the past decade, although there is no generic solution that can be applied to the full range of drugs or solid dosage forms. The present study provides a new concept, using the 3D printing technique to print a coating system in the form of shells with various

Permeable hollow 3D tissue-like constructs engineered by on-chip hydrodynamic-driven assembly of multicellular hierarchical micromodules

Engineered three-dimensional (3D) microtissues that recapitulate in vivo tissue morphology and microvessel lumens have shown significant potential in drug screening and regenerative medicine. Although microfluidic-based techniques have been developed for bottom-up assembly of 3D tissue models, the spatial organization of heterogeneous micromodules into tissue-specific 3D constructs with embedded microvessels remains challenging. Inspired by a hydrodynamic-based classic game which stacks rings

3D Printing of Cell-Laden Electroconductive Bioinks for Tissue Engineering Applications

Bioprinting is an emerging powerful fabrication method, which enables the rapid assembly of 3D bioconstructs with dispensing cell-laden bioinks in pre-designed locations. However, to translate this technology into real applications, there are still a number of challenges that need to be addressed. First, the current inks are generally composed of polymeric materials with poor electrical conductivity that mismatches with the

3D bioprinting of cell-laden electroconductive MXene nanocomposite bioinks

MXenes, a new family of burgeoning two-dimensional (2D) transition metal carbides/nitrides, have been extensively explored in recent years owing to their outstanding properties such as a large specific surface area, high electrical conductivity, low toxicity, and biodegradability. Numerous efforts have been devoted to exploring MXenes for various biomedical applications such as cancer therapy, bioimaging, biosensing, and drug delivery. However, the

Bioengineering Vascular Networks to Study Angiogenesis and Vascularization of Physiologically Relevant Tissue Models in Vitro

Angiogenesis assays are essential for studying aspects of neovascularization and angiogenesis and investigating drugs that stimulate or inhibit angiogenesis. To date, there are several in vitro and in vivo angiogenesis assays that are used for studying different aspects of angiogenesis. Although in vivo assays are the most representative of native angiogenesis, they raise ethical questions, require considerable technical skills, and are expensive. In vitro assays are inexpensive and

Bioprinting Cell- and Spheroid-Laden Protein-Engineered Hydrogels as Tissue-on-Chip Platforms

Human tissues, both in health and disease, are exquisitely organized into complex three-dimensional architectures that inform tissue function. In biomedical research, specifically in drug discovery and personalized medicine, novel human-based three-dimensional (3D) models are needed to provide information with higher predictive value compared to state-of-the-art two-dimensional (2D) preclinical models. However, current in vitro models remain inadequate to recapitulate the complex and heterogenous

3D printed cellulose-based high-efficiency oil-water separation mesh

In this study, a high-performance oil-water separation mesh based on cellulose was fabricated by Direct Ink Writing (DIW) 3D printing technology. The 3D printing process can be undergo in a natural environment by using a cellulose acetate/ethyl acetate solution as a DIW printing ink. This oil-water separation mesh prepared has various advantages, such as uniform size, controllable aperture, simple and

Robust pure copper framework by extrusion 3D printing for advanced lithium metal anodes

The application of lithium metal anodes is hindered by safety issues originating from unwanted Li dendritic growth. To address this issue, designing 3D porous Cu current collectors is an effective strategy to modify Li deposition behaviour. However, known fabrication processes for these porous collectors are usually complicated, expensive and non-scalable. There is also lack of investigation into their mechanical strength,

Investigating lymphangiogenesis in a sacrificially bioprinted volumetric model of breast tumor tissue

Lymphatic vessels, as a means to metastasize, are frequently recruited by tumor tissues during their progression. However, reliable in vitro models to dissect the intricate crosstalk between lymphatic vessels and tumors are still in urgent demand. Here, we describe a tissue-engineering method based on sacrificial bioprinting, to develop an enabling model of the human breast tumor with embedded multiscale lymphatic

Complexation-induced resolution enhancement of 3D-printed hydrogel constructs

Three-dimensional (3D) hydrogel printing enables production of volumetric architectures containing desired structures using programmed automation processes. Our study reports a unique method of resolution enhancement purely relying on post-printing treatment of hydrogel constructs. By immersing a 3D-printed patterned hydrogel consisting of a hydrophilic polyionic polymer network in a solution of polyions of the opposite net charge, shrinking can rapidly occur

Novel bioinks from UV-responsive norbornene-functionalized carboxymethyl cellulose macromers

3D printing has significantly progressed in the past decade and become a potentially powerful biomanufacturing approach for tissue and organ printing. Availability of diverse hydrogel-based bioink formulations, particularly bioinks allowing biochemical functionalization, stimuli responsiveness, and control over mechanical and degradation properties are crucial for bioprinting to reach its full potential. In this study, we report two novel bioink platforms from

Boosting the Osteogenic and Angiogenic Performance of Multiscale Porous Polycaprolactone Scaffolds by In Vitro Generated Extracellular Matrix Decoration

Tissue engineering (TE)-based bone grafts are favorable alternatives to autografts and allografts. Both biochemical properties and the architectural features of TE scaffolds are crucial in their design process. Synthetic polymers are attractive biomaterials to be used in the manufacturing of TE scaffolds, due to various advantages, such as being relatively inexpensive, enabling precise reproducibility, possessing tunable mechanical/chemical properties, and ease

Printing 3D Hydrogel Structures Employing Low-Cost Stereolithography Technology

Stereolithography technology associated with the employment of photocrosslinkable, biocompatible, and bioactive hydrogels have been widely used. This method enables 3D microfabrication from images created by computer programs and allows researchers to design various complex models for tissue engineering applications. This study presents a simple and fast home-made stereolithography system developed to print layer-by-layer structures. Polyethylene glycol diacrylate (PEGDA) and gelatin

3D Bioprinting of Methylcellulose/Gelatin-Methacryloyl (MC/GelMA) Bioink with High Shape Integrity

The advent of three-dimensional (3D) bioprinting offers a feasible approach to construct complex structures suitable for tissue regeneration, during which cell-laden materials are dispensed on a substrate according to a predesigned structure. However, the lack of ideal printable bioinks with high shape fidelity and improved biological stability remains a major challenge. In this study, methylcellulose/gelatin-methacryloyl (MC/GelMA) bioink with high shape

Immersion Bioprinting of Tumor Organoids in Multi-Well Plates for Increasing Chemotherapy Screening Throughput.

The current drug development pipeline takes approximately fifteen years and $2.6 billion to get a new drug to market. Typically, drugs are tested on two-dimensional (2D) cell cultures and animal models to estimate their efficacy before reaching human trials. However, these models are often not representative of the human body. The 2D culture changes the morphology and physiology of cells,

Inorganic Sol–Gel Polymerization for Hydrogel Bioprinting

An inorganic sol–gel polymerization process was used as a cross-linking reaction during three-dimensional (3D) bioprinting of cell-containing hydrogel scaffolds. Hybrid hydroxypropyl methyl cellulose (HPMC), with a controlled ratio of silylation, was prepared and isolated as a 3D-network precursor. When dissolved in a biological buffer containing human mesenchymal stem cells, it yields a bioink that can be printed during polymerization by

Easy-to-use and inexpensive sensors for assessing the quality and traceability of cosmetic antioxidants

We describe an easy-to-use sensor as a tool to quantify and authenticate antioxidant active ingredients in cosmetic products. The sensing platform comprises a responsive nanoengineered surface that reacts specifically and generates distinct optically detectable signals that are representative of the chemical composition and concentration of active ingredients. The platform can be inexpensively produced in large quantities and a procedure to