Changing Cell Media (HUVECs)

Share on linkedin
Share on facebook
Share on twitter
Share on reddit
Share on whatsapp
Share on linkedin
Share on facebook
Share on twitter
Share on reddit
Share on whatsapp
cell media changing cell media medium protcol allevi bioprinting

Overview

This protocol will walk you through the process of feeding HUVECs. 

Materials

Methods

  1. Warm EGM™-2 to 37˚C in the water/bead bath;
  2. Spray it down with ethanol and place them inside a biosafety cabinet (BSC);
  3. Spray down incubator doors with ethanol and minimize breathing to diminish contamination risk;
  4. Remove cell culture flasks from incubator and place them under microscope to ensure there is no contamination;
  5. Still, under the microscope, check for flask confluency by analyzing the ratio of surface area occupied by cells and surface area not occupied by cells. If confluency is ~70-85%, follow this protocol on cell passaging;
  6. Spray down flasks with ethanol, wipe them with kimwipes and place them in BSC;
  7. Aspirate cell media from culture flasks, ensuring to avoid touching the bottom of the flask;
  8.  Add fresh cell medium to your flasks;
    • Note: if your cells are 25-45% confluent, add 0.3 mL/cm2. If your cells are 45-70% confluent, add 0.4 mL/cm2;
  9.  Place your flasks back into the incubator;
  10.  Feed every 48 hours.

Share this article

Share on linkedin
Share on facebook
Share on twitter
Share on reddit
Share on whatsapp