Coating Plates with PureCol®

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scientist in lab Coating Plates with PureCol®

Overview

Cells respond to a variety of stimuli present in their culture components such as media and surfaces in which they are cultured. Depending on the application and cell line, coating your dish with PureCol® will enable better cell attachment and function. This protocol walks you through the process of coating plates with PureCol® (Type I collagen).  

Materials

Methods for Coating Plates with PureCol®

  1. Using a pipette, transfer your desired quantity of PureCol® from the stock bottle to a centrifuge tube;
  2. Dilute PureCol® with sterile water or 0.01 N HCl in a 1:30 proportion;
  3. Gently rock the tube to mix contents;
    • Note: Do NOT vortex this material.
  4. Add the appropriate amount of diluted PureCol to your dish of choice. Refer to the table below for recommendations per dish;
    • Note: Ensure that your surface is fully coated.
PlateWell Working Volume
6-well2 mL
12-well1 mL
24-well500 µL
48-well200 µL
96-well100 µL
  1. Cover your plate and incubate it at room temperature until the surface is dry (1-2 hours).
  2. Rinse surfaces with PBS or sterile medium;
  3. Your dish is ready to use! You may also store it in 2-10˚C, ensuring to maintain sterility.

We hope this protocol helps you with coating plates with PureCol® (Type I collagen).  Click here for more bioink preparation protocols.

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