Cells respond to a variety of stimuli present in their culture components such as media and surfaces in which they are cultured. Depending on the application and cell line, coating your dish with PureCol® will enable better cell attachment and function. This protocol walks you through the process of coating plates with PureCol® (Type I collagen).
- Sterile Water or 0.01 N HCl Solution
- Sterile PBS or Medium
- Dish of Choice
- Centrifuge Tubes
- Micropipette Tips
Methods for Coating Plates with PureCol®
- Using a pipette, transfer your desired quantity of PureCol® from the stock bottle to a centrifuge tube;
- Dilute PureCol® with sterile water or 0.01 N HCl in a 1:30 proportion;
- Gently rock the tube to mix contents;
- Note: Do NOT vortex this material. Vortexing will cause bubble formation.
- Add the appropriate amount of diluted PureCol to your dish of choice. Refer to the table below for recommendations per dish;
- Note: Ensure that your surface is fully coated.
|Plate||Well Working Volume|
- Cover your plate and incubate it at room temperature until the surface is dry (1-2 hours).
- Rinse surfaces with PBS or sterile medium;
- Your dish is ready to use! You may also store it in 2-10˚C, ensuring to maintain sterility.
We hope this protocol helps you with coating plates with PureCol® (Type I collagen). Click here for more bioink preparation protocols.