If in need of a refresher, review this aseptic technique video.
Remember to turn on biosafety cabinet at least 15 minutes before use. Wipe down with kim wipes and 70% ethanol.
Be sure to generously spray everything to be placed in biosafety cabinet with 70% ethanol. Allow your gloves and other materials to dry to give ethanol time to kill bacteria and other contaminants (trick: sing happy birthday to yourself before using anything you have sprayed in).
Do not pass your hands or anything else over any open containers. Minimize the number of containers that are open. To help avoid this work from right to left (if right-handed) or left to right (if left-handed).
Place caps bottom up towards the back of the biosafety cabinet.
Minimize waste and other supplies in the biosafety cabinet to minimize disruption of airflow.
When pipetting, if pipette touches anything outside of a sterile container, use a new pipette. When in doubt, use a new pipette. When changing media, change pipettes at a minimum of every 3 – 4 flasks.