Glass Slide Treatment

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Print surfaces in bioprinting can have a large impact on final results [1]. This glass slide treatment can be used for printing with GelMA or PEGDA to keep structures adhered to a specific surface, allowing for improved maneuvering and control over printed structures.

You Will Need:

Protocol:

  1. First, sonicate glass slides for 5 minutes in ethanol.
  2. Replace with new ethanol solution and sonicate again for another 5 minutes.
  3. Sonicate glass slides in deionized water.
  4. Under a chemical hood, prepare a solution of 5% TMSPMA (v/v) in toluene.
  5. Soak slides in prepared solution overnight at 60°C.
  6. Remove slides from TMSPMA solution and wash with two ethanol rinses and two deionized water rinses.
  7. Sterilize slides in ethanol before use with cells.

References:

[1] Jia Min Lee & Wai Yee Yeong (2015) A preliminary model of time-pressure dispensing system for bioprinting based on printing and material parameters, Virtual and Physical Prototyping, 10:1, 3-8

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