Bioprinting Hard Tissues Using PLGA

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Bioprinting Hard Tissues Using PLGA

Overview

Poly(lactic-co-glycolic acid), or PLGA, is a common thermoplastic used in bioprinting for its tunable degradable and mechanical properties. The degradation and mechanical properties of this material can be altered by adjusting the ratios of the copolymers polylactide and polyglycolide. Thermoplastics in bioprinting are often used as a support structure to provide extra mechanical strength for hard tissues and as reinforcement for matrix bioinks. Follow this step-by-step protocol for bioprinting hard tissues using PLGA.

Materials

Methods for Bioprinting Hard Tissues Using PLGA

  1. Load PLGA into a metal syringe;
  2. Screw the metal tip on the syringe;
    • Note: we suggest using a 27G for best resolution or a 19G for the fastest printing.
  3. Set your Allevi CORE™ extruder temperature to 100˚C;
  4. Load your syringe into the extruder;
  5. Wait for approximately 20 min until the PLGA melts before starting to print.

Print Settings: PLGA Protocol

Speed (mm/s)Layer height (mm)Nozzle Diam (mm)  Gauge
1.00.10.130
Pressure (psi) Crosslink (sec)Print Temp (°C)
1000100

Note: different gauges require different pressure and yield different resolutions.

bioprinting hard tissues PLGA - PLGA lattice, cylinder, and lines
Fig 1: PLGA lattice (left), cylinder (middle), and line (right) structures printed with the Allevi 2.

We hope you found this protocol helpful for bioprinting hard tissues using PLGA! Click here for more bioprinting protocols.

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