Poly(lactic-co-glycolic acid), or PLGA, is a common thermoplastic used in hard tissue engineering . The recommended preparation provided in the user instruction below is a good starting point for bioprinting PLGA.
Storage and Handling
For long term storage, PLGA should be kept at -20ºC in an airtight container.
You Will Need
- Metal needles
- Petri dish or well plate for printing
- Stainless Steel Allevi Syringe (5mL or 10mL)
- Suggested: painter’s tape for improved first layer adhesion
Instructions for Use
*If printing with cell-laden matrix bioinks, all steps must be completed in a sterile environment. Be sure to sterilize PLGA overnight prior to use with cells.
- If using with cells, first sterilize PLGA.
- Measure the desired amount of PLGA for printing and load into metal syringe.
- Cap the metal syringe with a metal needle.
- Load syringe into the first extruder in the Allevi printer. Connect to your Allevi printer through your computer.
- Set the heat for the first extruder to suggested print temperature and allow time for PLGA to melt.
- Optional: To help with adhesion of the material, painter’s tape is suggested as a printing surface.
|Speed (mm/s)||Layer Height (mm)||Nozzle Diam (mm)||Gauge||Pressure (PSI)||Print Temp (°C)|
|1.0||0.2||0.15||30 Metal Tapered||100||95|
Try a practice print by following the instructions for Your First Bioprint using the print parameters and loading instructions for PLGA.
- Print speed, nozzle diameter and pressure may all be adjusted to better fit a user’s needs. For help adjusting print parameters please contact [email protected].
- A fill volume change of more than 2 ml may affect pressure settings.
- A lower gauge size or tapered gauge will require lower pressure, while a higher gauge will require higher pressure for extrusion. Lowering the gauge size will also generally lower resolution.
 Pati, Falguni et al, “Ornamenting 3D Printed Scaffolds with Cell-laid Extracellular Matrix for Bone Tissue Regeneration” Biomaterials, 2015(37), pp. 230 – 241.