Bioprinting Hard Tissues Using PLGA

Bioprinting Hard Tissues Using PLGA


Poly(lactic-co-glycolic acid), or PLGA, is a common thermoplastic used in bioprinting for its tunable degradability and mechanical properties. The degradation and mechanical properties of this material can be altered by adjusting the ratios of the copolymers polylactide and polyglycolide. Thermoplastics in bioprinting are often used as a support structure to provide extra mechanical strength for hard tissues and as reinforcement for matrix bioinks. Follow this step-by-step protocol for bioprinting hard tissues using PLGA.



  1. Load PLGA into a metal syringe;
  2. Screw the metal tip on the syringe;
  3. Set your Allevi CORE™ extruder temperature to 100˚C;
  4. Load your syringe into the extruder;
  5. Wait for approximately 20 min until the PLGA melts before starting to print.

Print Settings: PLGA Protocol

Speed (mm/s)Layer height (mm)Nozzle Diam (mm)  Gauge
Pressure (psi) Crosslink (sec)Print Temp (°C)

Note: different gauges require different pressure and yield different resolutions.

bioprinting hard tissues PLGA - PLGA lattice, cylinder, and lines
Fig 1: PLGA lattice (left), cylinder (middle), and line (right) structures printed with the Allevi 2.

We hope you found this protocol helpful for bioprinting hard tissues using PLGA! Click here for more bioprinting protocols.

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