Collagen type I is the most abundant protein in the extracellular matrix and has been widely used in academia and industry for tissue engineering applications. With Allevi’s exclusive CORE™ printhead and the Tissue Layering Kit, you are now able to print and pattern pure 3 mg/mL type I collagen or 8 mg/mL methacrylated collagen. This is the first time that such low concentration collagen can be printed and patterned through 3D bioprinting.
Storage and Handling
Collagen should be stored 4˚C.
You Will Need
- 10 mL of Advanced Biomatrix PureCol
- Allevi 5 mL syringe
- 2 x Allevi layering tips™
- Syringe coupler
- 10X PBS
- Cell media
All items come sterile.
Instructions for Use
- Add 1 part of chilled 10X PBS to 8 parts of Advanced Biomatrix PureCol;
- Collagen should be maintained at a temperature of 4˚C to prevent premature crosslinking;
- Adjust the pH of the mixture to 7 – 7.5 by adding sterile 0.1 M NaOH;
- Add water to complete full volume to 10 parts;
- Mix cells with collagen by pipetting collagen onto cell pellet;
- With the Allevi syringe, draw 1.5 mL of your cell solution.
- Set your Allevi extruder to 4˚C;
- Attach the Allevi layering tip™ to your syringe;
- Bioprint on the Allevi printing dish;
- If you have an A3, set your bed plate to 37˚C to crosslink your skin layers at the time of deposition;
- Incubate your structure at 37˚C for at least 30 minutes;
- Culture your layered tissue as desired.
|Speed (mm/s)||Layer height (mm)||Nozzle Diam (mm)||Gauge|
|Pressure (PSI)||Crosslink (sec)||Print Temp (°C)|
Lee, V., Singh, G., Trasatti, J. P., Bjornsson, C., Xu, X., Tran, T. N., … Karande, P. (2014). Design and Fabrication of Human Skin by Three-Dimensional Bioprinting. Tissue Engineering Part C: Methods, 20(6), 473–484. https://doi.org/10.1089/ten.tec.2013.0335