Pluronic is a sacrificial bioink that can be used for temporary support or to create channels, vessel or vasculature for bioprinting purposes. The recommended preparation provided in the user instruction below yields a streamlined printable biomaterial that supports 3D printed cellular constructs. However, preparation can be modified by users to suit their needs.
Storage & Handling
Pluronic should be stored at 4°C until ready for use.
Instructions for Use
- Remove Pluronic F127 Syringe from storage.
- Allow Pluronic F127 to warm to room temperature.
- Print construct.
- To remove Pluronic F127 from printed construct, cool entire construct to 4°C over ice. Use a vacuum if necessary to remove material.
|Speed (mm/s)||Layer Height (mm)||Nozzle Diameter (mm)||Gauge||Pressure||Print Temp(C)|
|6-10||0.2||0.2*||0.25″ straight 30||80||Room temperature|
*Note that the nozzle diameter print setting for this material is slightly larger than the diameter of the recommended gauge (150 μm).
Want to try a Practice Print?
Check out the information for Your First Bioprint for further instructions on preparing files for printing and general use instructions for Pluronic F127.
- For help adjusting print parameters please contact [email protected]
- A fill volume change of more than 2 ml may affect pressure settings.
- A lower gauge size or tapered gauge will require lower pressure, while a higher gauge will require higher pressure for extrusion. Lowering the gauge size will also generally lower resolution
 D. B. Kolesky et al, “3D Bioprinting of Vascularized, Heterogeneous Cell-laden Tissue Constructs,” Adv. Mater., vol. 26, pp. 3124-3130, 2014.