Collagen type I is the most abundant protein in the extracellular matrix and has been widely used in academia and industry for tissue engineering applications. With Allevi’s exclusive CORE™ printhead and this bioprinting collagen protocol, you are now able to print and pattern pure 3 mg/mL type I collagen or 8 mg/mL methacrylated collagen for layered tissues such as skin. This is the first time that such low concentration collagen can be printed and patterned through 3D bioprinting.
- Advanced Biomatrix PureCol®
- Allevi 5 mL Syringe
- Allevi Layering Tips
- Syringe Coupler
- 10X PBS
- Cell Media
- Follow this protocol to prepare PureCol® and your cells for printing;
- Set your Allevi extruder temperature to 4˚C;
- Attach the Allevi layering tip™ to your syringe;
- Bioprint on the Allevi printing dish;
- If you have an A3, set your bed plate to 37˚C to crosslink your collagen layers at the time of deposition;
- Incubate your structure at 37˚C for at least 30 minutes;
- Culture your layered tissue as desired.
|Speed (mm/s)||Layer height (mm)||Nozzle Diam (mm)||Gauge|
|Pressure (PSI)||Crosslink (sec)||Print Temp (°C)|
We hope you found this protocol for bioprinting collagen for layered tissues helpful! You are now ready for imaging and analysis of your layered tissue construct – click here for analysis protocols.