How to Bioprint a Buckyball Using Fresh

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Below are print files and instructions for this sample buckyball print structure. Print settings are for a 2% sodium alginate solution.

Files

  • STL
  • Gcode
  • Slicing Parameters

Print Settings

If beginning with the gcode file, you can simply upload the file directly to the Allevi software and print! Use these suggested print settings to fabricate this structure with the FRESH method using 2% (w/v) sodium alginate. You can learn more about how to edit and use gcode files here.

Speed (mm/s)Layer Height (mm)Nozzle Diam (mm)GaugePressure (psi)Print Temp (C)
6.00.20.230 1″7-825

File Preparation

If beginning with the .STL file, first download the file and load it into Repetier-Host.

  • Next, adjust the size of the file by scaling it down to 0.25
  • Upload the slicing parameters to slic3r. From these starting parameters, you can adjust needle size, layer height and print speed.

To load the config (.ini) file, open slic3r settings from repetier host and then go to file > load config.

  • Slice and then save your gcode file
  • Upload your file to the Allevi Software and print!

You can find more information about FRESH printing method here. FRESH bioprinting supplies are available in our shop.

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