PEGDA Bioprinting

PEGDA Bioprinting


Poly(ethyelene glycol) diacrylate, or PEGDA, is a PEG-based hydrogel that reacts and crosslinks in the presence of LAP photoinitiator and Allevi blue light technology. The recommended preparation provided in the user instruction below yields a streamlined matrix bioink that supports 3D printed cell-laden constructs. However, preparation can be modified by users to suit their needs. Follow this step-by-step process for PEGDA bioprinting.

Note: Due to low viscosity, PEGDA solutions can be difficult to work with when bioprinting. For optimal results, we suggest mixing this reagent with a more viscous material.



  1. Measure 0.8% w/v LAP concentration and mix in cell media or PBS at 60°C in a light-protected vial. Mix until all LAP is dissolved.
  2. Measure 33.33% w/v PEGDA concentration and add to the solution, still in a light-protected vial. Mix at 60°C until all PEGDA is dissolved.
  3. Adjust pH of solution from step 2 to 7.4.
  4. Filter solution with a 0.45 µm syringe filter under sterile conditions.*
  5. Mix solution with Percoll to create a 40% (v/v) Percoll solution.
    • Note: For 5 ml, you will need 3 ml of solution and 2 ml of Percoll.
  6. Mix in desired cell concentration in a sterile container.*
  7. Load cell-laden bioink into a sterile plastic syringe.
  8. Load syringe into Allevi bioprinter.
  9. Place the desired printing dish onto the print-bed.
  10. Design or upload your CAD file in the Allevi software and use the print settings outlined below. Print your desired structure.
    • Note that this material will need to be combined with some type of support or sacrificial reagent such as pluronic or PCL.

* For prints without cells, step 4 & step 6 may be skipped.

Print Settings

Speed (mm/s) Layer Height (mm) Nozzle Diam (mm) Gauge Pressure (psi) Print Temp (C) tapered3-525

Post-print settings

Crosslinking Time (s) Crosslink Intensity (mW/cm^2)
240 10


  1. Gelation time and gel stiffness can be adjusted by varying the concentration of PEGDA or LAP. For help adjusting print parameters please contact [email protected].
  2. A fill volume change of more than 2 ml may affect pressure settings.
  3. A lower gauge size or tapered gauge will require lower pressure, while a higher gauge will require higher pressure for extrusion. Lowering the gauge size will also generally lower resolution

We hope that you found this step-by-step guide for PEGDA bioprinting to be helpful! You are now ready to analyze and image your 3D bioprinted structure – click here for post-print analysis protocols.


B. D. Fairbanks et. al, “Photoinitiated Polymerization of PEG-diacrylate with lithium phenyl-2,4,6-trimethylbenzoylphosphinate: polymerization rate and cytocompatibility,” Biomaterials, vol. 30, no. 35, pp. 6702-6707, Dec 2009.

Chan V et al. “Three-dimensional photopatterning of hydrogels using stereolithography for long-term cell encapstulation.” Lab Chip. 2010(10) 2062-70.

Lin, Hang et al. “Application of Visible Light-based Projection Stereolithography for Live Cell-Scaffold Fabrication with Designed Architecture.” Biomaterials 2013(34). pp. 331- 39.

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