Quickstart Guide: Allevi 3

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Allevi 3 bioprinter - quickstart guide: allevi 3

Strong and capable. The Allevi 3 delivers autocalibration, 4-160°C temperature control, and visible/UV photocuring in three independent printheads. Find your bioink of choice and start building. This quickstart guide of manuals and videos will help you get started with your new Allevi 3 bioprinter.

Specifications

View Allevi 3 specifications.

Setting up your bioprinter

  1. Carefully remove your printer from the packaging by grasping the printer frame firmly with both hands.
    • CAUTION: The Allevi 3 weighs ~48lbs (22kg). Two people may be required to safely lift it.
  2. Once the printer is securely placed on a flat surface, connect the CORE extruders to the gantry. Each CORE extruder is magnetized so each will snap into place.
  3. Connect the red plastic air hoses to the top of the frame, and the air input junction to the port on the back of the frame.
  4. Plug the power cable into the power port located on the right side of the back of the bioprinter. Then plug the other side into a wall outlet.
  5. Power on the bioprinter by pressing the power button located on front of the bioprinter. It will turn red.
  6. Connect the air compressor to the black air hose, and the air hose to the air input junction on the printer.
    • The connection between the hose and the junction can be tricky. Follow this diagram:

A. Gather both the air input junction (left) and the air hose (right).
B. Pull back the collar of the air hose.
C. Insert the air input junction in the air hose.
D. Slide the collar of the air hose back into place.
E. The connection should be formed!
F. Give the connection a light tug to ensure it is secure.

The air compressor must remain turned on for the entirety of the print. We recommend keeping the air compressor set to a pressure significantly higher than your print pressure. This is so that as the air compressor loses some pressure and refills, your print is not affected. The air compressor’s pressure can be adjusted by turning the black knob. It has a max pressure of 120PSI.

quickstart guide: allevi 3

Setting up your software

  1. Go to bioprint.allevi3d.com. Our software runs out of a browser and requires no downloads! We recommend Google Chrome or Firefox, but Safari and Edge are also acceptable.
  2. Login to the software. To set up an account, click ‘Create an account’ and fill out the form.
  1. Creating an account is great, but let’s get you set you up to add some teammates! Follow our Allevi Teams Guide to create a Team. We recommend doing this first so that your printers and files are easily shared with others.
  2. As you follow the rest of these instructions, make sure your Team account is active, rather than your Personal account.
  3. To register your printer, scroll down in your printer list and click ‘Add printer.’
  1. Type in your device serial number and click ‘Add printer.’ Your serial number can be found on the back of your printer or in your onboarding email from the Allevi Support Team. If someone on your Team has already registered your printer, contact Allevi Support to gain access.
  1. Once your printer is registered, it will appear as an available printer in your printer list. You can also see its Internet connection status.
  1. Connect your Allevi bioprinter to the Internet. You can do this either with ethernet (wired) or WiFi (wireless).
    1. Open the WiFi settings on your computer and connect to the network called “Allevi WiFi Connect.” After connecting to it, the WiFi Dashboard should pop up automatically. If it does not, go to 192.168.42.1 in your browser. By connecting to Allevi WiFi Connect, your computer will briefly lose access to the Internet.
    2. Copy down your wlan0 and etho0 MAC addresses. These are important if your IT department requires device registration.
    3. To connect via ethernet, plug the ethernet cord into the ethernet port on the back of the Allevi 3 and plug the other side into a working ethernet outlet on your wall.
    4. To connect via WiFi, choose your WiFi network from the dropdown menu under ‘SSID,’ type in the password, and hit connect.
      • NOTE: The chosen WiFi network should be a private network without a ‘Click to accept’ intermediate page.
    5. Your computer’s WiFi will be restored and your printer will connect to the Internet.

Running a print

The Allevi Bioprint Online software is the most comprehensive and versatile bioprinting software. We recommend watching this introduction video.

  1. With your Allevi 3 turned on and connected to the Internet, you can get started by clicking connect!
    • NOTE: Upon turning on your Allevi 3, all extruder will retract.
choose your bioprinter - getting started with Allevi 3
  1. This will bring you to your printer Status section. Here you can get an overview of your print settings.
quickstart guide: allevi 3
  1. The next section is your Current Print section. You can either upload STL or gcode files. If you upload an STL, you can modify the Allevi Online Slicer settings. For STL files, you can changes settings such as Print Settings, Infill, and Offsets. You can also download a sliced STL as a gcode file.
  1. Next, choose your dish in the Build Plate section. For wellplates, print files will automatically be replicated in all wells.
  1. The Extruders section allows you to move and calibrate your extruders. You can also set extruder temperature, pressure, and test extrude by holding the button. With a syringe and needle loaded, click ‘Autocalibrate’ to get started. All attached extruders must be calibrated in order to print.
extruder parameters - quickstart guide: allevi 3
  • To manually calibrate the printer, open the jog panel:
    1. Click the origin button under the X/Y controls. This will center your extruder over your chosen build plate.
    2. Using the Z controls, move the bedplate closer to the loaded extruder to the point where the dish is almost touching the needle.
      • NOTE: Use the test extrude button to see if the needle is properly positioned. If the needle is too low, no material will come out. If it is too high, then a dangling line will be extruded.
      • Under extrusion, the needle should just touch the dish. Do not leave a gap between the needle and dish as the gcode automatically adds the appropriate layer height. Finer 0.1mm and 0.05mm movement can be used when approaching the dish.
    3. Click ‘Set Calibration’ to save your position as the calibration.
  1. In the Crosslinking section, there are three main settings: Always On, During Print, and Post Print. They use variables such as light intensity, layer frequency, and duration. For photocurable bioinks, the Allevi 3 offers both UV (365nm) and Visible (405nm) light crosslinking. To control a certain extruder’s crosslinking settings, make sure you select that extruder in the Extruders tab first.
crosslinking parameters quickstart guide: allevi 3
  1. The Print Bed section is where you control the print bed temperature, which ranges from room temperature to 60°C. For more even heating, use the metal inserts that came with your printer.
  1. Once you’ve loaded a print file and calibrated your extruders, you can click ‘Print’ to start building with life!
quickstart guide: allevi 3 - test bioprint

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