Bioprinting Parameters for Every Bioink Cheat Sheet

Welcome to the Allevi quick start guide to bioprinting parameters for every bioink! The following bioprinting parameters are general guidelines for bioinks offered on the Allevi platform, but with some simple experiments, you can optimize them for your exact application!

Every bioink requires different parameters in order to achieve high printability and viability. The slicing parameters should be entered into Repetier-Host when slicing your STL file. Print parameters can be set in the software.

Bioink Bioprinting Parameters Guide:

BioinkLayer Height (mm)Speed (mm/s)Pressure (PSI)Temperature (°C)NeedleCrosslinking
Alginate (2%)0.1567.5RT1.0″ straight 30GChemical (CaCl2)
Alginate (5%)0.15615RT1.0″ straight 30GChemical (CaCl2)
Carbohydrate Glass0.2860110Tapered metal 27GN/A
Gelatin0.36-1010-20RTTapered metal 27GN/A
Gelatin Methacrylate0.36-1010-20RTTapered metal 27GPhoto (LAP, 410nm)
Graphene0.210-1530-40RTTapered metal 27GN/A
Hyperelastic Bone0.210-1530-40RTTapered metal 27GN/A
Collagen Lifeink2000.264041.0″ straight 30GThermal
Collagen Lifeink200 (with cells)0.261541.0″ straight 30GThermal
Matrigel0.156540.25″ straight 30GThermal
PCL0.11-2100100Tapered metal 30GN/A
PEGDA0.343-525Tapered metal 30GPhoto (LAP, 410nm)
PLGA0.21100100Tapered metal 30GN/A
Pluronic0.26-1580RT0.25″ straight 30GN/A

We hope that these bioprinting parameters help you get started with your bioink of choice. If you’re working with a custom bioink, follow our guide on How to Optimize a CustomBioink, or read our article on the 8 Crucial Steps for Print Parameter Optimization.

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