The Allevi Blog

Passaging Human Mesenchymal Stem Cells (hMSCs)

Overview Passaging is required when human mesenchymal stem cells have proliferated to confluences around 90%. This important step enables you to culture enough cells for your experiments, as well as

Thawing Human Mesenchymal Stem Cells (hMSCs)

Overview Thawing cells properly is crucial to cell culture health. Follow this protocol for thawing cryopreserved human mesenchymal stem cells to ensure your cells will have an optimal yield for

Thawing Human Dermal Fibroblasts (Neonatal or Adult)

Overview Thawing cells properly is crucial to cell culture health. Follow this protocol to ensure your cryopreserved human dermal fibroblasts will have an optimal yield for your experiments. Materials Human

Encapsulating Hepatocytes

Overview Human hepatocytes can be encapsulated in various bioinks for printing liver organoids or other geometries. Follow this protocol to encapsulate your hepatocytes. Please note that these hepatocytes cannot be

Plating Hepatocytes

Overview Human hepatocytes can be plated, cultured and analyzed for different drug interactions or other liver function experiments. Follow this protocol to ensure your cells will have optimal yield and

Thawing Hepatocytes

Overview Thawing cells properly is crucial to cell culture health. Follow this protocol to ensure your cryopreserved hepatocytes will have an optimal yield for your experiments. Please note that these

Making Spheroids (Pipette Method)

Overview Spheroids are significant 3D in vitro models often used as a more physiologically relevant alternative to 2D cell culture. For an automated and standardized method of making spheroids, follow

Using Trypan Blue to Check Cell Viability

Overview When preparing cells for an experiment, it is useful to know what percentage of them are alive. This protocol will walk you through the process of checking cell viability

Preparing a Cell Pellet

Overview Preparing a cell pellet is a necessary step for a variety of experimental methods, including encapsulating cells in a bioink, cell counting and cell passaging. This protocol will walk

Hemocytometer Cell Counting

Overview There are a few ways in which you can count cells to prepare them for passaging or other experiments. Though automated cell counters save time when quantifying cell numbers,