The Allevi Blog

Print surfaces in bioprinting can have a large impact on your final results [1]. This glass slide treatment protocol can be used for bioprinting with GelMA or PEGDA to keep

Overview Gelatin is a sacrificial bioink that can be used for temporary support or to create channels, vessels, or vasculature for bioprinting purposes. Through reversible thermal gelation, gelatin remains solid

Overview Follow this protocol to learn how to mix native protein additives with bioinks for enhanced cellular response in 3D bioprinting applications. Materials Allevi plastic syringes (5 mL or 10

Overview Pluronic is a sacrificial bioink that can be used for temporary support or to create channels, vessels or vasculature for 3D bioprinting purposes. The recommended preparation provided in the

Overview PCL (polycaprolactone) is a thermoplastic polymer that allows for enhanced control over the mechanical properties of 3D printed structures. It is specifically developed and optimized for bioprinting of 3D

Overview Poly(ethyelene glycol) diacrylate, or PEGDA, is a PEG-based hydrogel that reacts and crosslinks in the presence of LAP photoinitiator and Allevi blue light technology. The recommended preparation provided in

Overview Poly(lactic-co-glycolic acid), or PLGA, is a common thermoplastic used in bioprinting for its tunable degradability and mechanical properties. The degradation and mechanical properties of this material can be altered

Introduction Follow these step-by-step instructions to perform an ATP quantification for 3D bioprinted constructs. This protocol uses a luminescent assay to determine the number of viable cells in culture. Note:

Introduction Follow these step-by-step instructions to perform a Live/Dead analysis on your 3D bioprinted constructs. This protocol uses a fluorescent assay to determine the number of viable cells in culture.

Overview Collagen type I is the most abundant protein in the extracellular matrix and has been widely used in academia and industry for tissue engineering applications. With Allevi’s exclusive CORE™

Overview LifeSupport® is the core component and industry standard for Freeform Reversible Embedding of Suspended Hydrogels (FRESH) 3D bioprinting. Each LifeSupport printing kit comes with five 2 g units of

Overview The Allevi Coaxial kit allows users with an Allevi 2 or Allevi 3 bioprinter to mix materials from two syringes during printing. This is especially useful when working with