The Allevi Blog

Bioprinting GelMA

Overview Materials Methods Print Settings Speed (mm/s) Layer height (mm) Nozzle diam (mm) Gauge Pressure (PSI) Print temp (˚C) 5 0.45 0.28 PT25-1.25 12-22 22 5 0.45 0.20 PT27-1.25 18-28

Multi-Extruder Bioprints

Below are directions for a sample dual extrusion bioprint with Bioprint software. This protocol is meant for educational purposes to help you learn how to use your Allevi 2 and

Solubilized PCL

Overview PCL (polycaprolactone) is a thermoplastic polymer that offers enhanced control over mechanical properties of final 3D structures. It is specifically developed and optimized for bioprinting of 3D structures. PCL

Silicone Bioprinting Protocol

Overview: Silicone Bioprinting This protocol is a step-by-step guide to bioprinting silicone. Silicones are a class of elastomers composed of polymerized siloxanes that are characterized by their high durability, low

Triaxial Bioprinting Protocol

Overview The Allevi Triaxial Kit allows users to mix materials from three syringes during printing. Triaxial bioprinting allows for a new level of complexity and during-print material mixing. This is

Loading Viscous Materials for Bioprinting

Loading viscous materials, such as silicone or some formulations of collagen, might be tricky. If you need to transfer a viscous biomaterial to a printing syringe follow these simple steps:

gelatin methacrylate gelMA lyophilized allevi bioink

How to Prepare GelMA Bioink (Lyophilized)

Overview Gelatin Methacrylate (GelMA) is a hydrogel composed of denatured collagen that is engineered to crosslink when combined with a photoinitiator, such as LAP. This matrix material is a highly


Immunocytochemistry for 3D Bioprinted Constructs

Overview Immunocytochemistry (ICC) is a laboratory technique that allows for the detection of specific proteins or antigens in cells using selective probes that can be visualized under a microscope. This

Organ-on-a-chip Bioprinting Using Pluronic

Overview Organs-on-chips have proved to be extremely valuable systems utilized for disease modeling, drug testing, and mimicking organ function. This organ-on-a-chip protocol allows you to build structures with controlled inner