The Allevi Blog

Bioprinting GelMA

Overview GelMA (gelatin methacrylate) is a gelatin-based hydrogel composed of denatured collagen that reacts and crosslinks in the presence of LAP and UV (365 nm) light (1,2). The recommended preparation

Multi-Extruder Bioprints

Below are directions for a sample dual extrusion bioprint with Bioprint software. This protocol is meant for educational purposes to help you learn how to use your Allevi 2 and

Solubilized PCL

Overview PCL (polycaprolactone) is a thermoplastic polymer that offers enhanced control over mechanical properties of final 3D structures. It is specifically developed and optimized for bioprinting of 3D structures. PCL

Silicone Bioprinting Protocol

Overview: Silicone Bioprinting This protocol is a step-by-step guide to bioprinting silicone. Silicones are a class of elastomers composed of polymerized siloxanes that are characterized by their high durability, low

Triaxial Bioprinting Protocol

Overview The Allevi Triaxial Kit allows users to mix materials from three syringes during printing. Triaxial bioprinting allows for a new level of complexity and during-print material mixing. This is

Loading Viscous Materials for Bioprinting

Loading viscous materials, such as silicone or some formulations of collagen, might be tricky. If you need to transfer a viscous biomaterial to a printing syringe follow these simple steps:


Immunocytochemistry for 3D Bioprinted Constructs

Overview Immunocytochemistry (ICC) is a laboratory technique that allows for the detection of specific proteins or antigens in cells using selective probes that can be visualized under a microscope. This

Organ-on-a-chip Bioprinting Using Pluronic

Overview Organs-on-chips have proved to be extremely valuable systems utilized for disease modeling, drug testing, and mimicking organ function. This organ-on-a-chip protocol allows you to build structures with controlled inner

Bioprinting Collagen for Layered Tissues

Overview Collagen type I is the most abundant protein in the extracellular matrix and has been widely used in academia and industry for tissue engineering applications. With Allevi’s exclusive CORE™