The Allevi Blog

Silicone Bioprinting Protocol

Overview: Silicone Bioprinting This protocol is a step-by-step guide to bioprinting silicone. Silicones are a class of elastomers composed of polymerized siloxanes that are characterized by their high durability, low

Triaxial Bioprinting Protocol

Overview The Allevi Triaxial Kit allows users to mix materials from three syringes during printing. Triaxial bioprinting allows for a new level of complexity and during-print material mixing. This is

Loading Viscous Materials for Bioprinting

Loading viscous materials, such as silicone or some formulations of collagen, might be tricky. If you need to transfer a viscous biomaterial to a printing syringe follow these simple steps:

How to Prepare GelMA Bioink (Sterile-Solution)

Overview GelMA is a highly used material in bioprinting due to its mechanical properties and printability. However, methacrylating gelatin in the lab is an inconveniently time-consuming process. In addition, buying

gelatin methacrylate gelMA lyophilized allevi bioink

How to Prepare GelMA Bioink (Lyophilized)

Overview Gelatin Methacrylate (GelMA) is a hydrogel composed of denatured collagen that is engineered to crosslink when combined with a photoinitiator, such as LAP. This matrix material is a highly


Immunocytochemistry for 3D Bioprinted Constructs

Overview Immunocytochemistry (ICC) is a laboratory technique that allows for the detection of specific proteins or antigens in cells using selective probes that can be visualized under a microscope. This

Organ-on-a-chip Bioprinting Using Pluronic

Overview Organs-on-chips have proved to be extremely valuable systems utilized for disease modeling, drug testing, and mimicking organ function. This organ-on-a-chip protocol allows you to build structures with controlled inner

Bioprinting Collagen for Layered Tissues

Overview Collagen type I is the most abundant protein in the extracellular matrix and has been widely used in academia and industry for tissue engineering applications. With Allevi’s exclusive CORE™

Bioprinting Liver Tissue

Overview Liver tissue function is determined by the interaction of cells with their microenvironment, namely through the interpretation of biochemical and mechanical signals present in its tissue-specific extracellular matrix. Hence,