The Allevi Blog

Triaxial Bioprinting Protocol

Overview The Allevi Triaxial Kit allows users to mix materials from three syringes during printing. Triaxial bioprinting allows for a new level of complexity and during-print material mixing. This is

Loading Viscous Materials for Bioprinting

Loading viscous materials, such as silicone or some formulations of collagen, might be tricky. If you need to transfer a viscous biomaterial to a printing syringe follow these simple steps:

How to Prepare GelMA Bioink (Sterile-Solution)

Overview GelMA is a highly used material in bioprinting due to its mechanical properties and printability. However, methacrylating gelatin in the lab is an inconveniently time-consuming process. In addition, buying

gelatin methacrylate gelMA lyophilized allevi bioink

How to Prepare GelMA Bioink (Lyophilized)

Overview Gelatin Methacrylate (GelMA) is a hydrogel composed of denatured collagen that is engineered to crosslink when combined with a photoinitiator, such as LAP. This matrix material is a highly


Immunocytochemistry for 3D Bioprinted Constructs

Overview Immunocytochemistry (ICC) is a laboratory technique that allows for the detection of specific proteins or antigens in cells using selective probes that can be visualized under a microscope. This

Organ-on-a-chip Bioprinting Using Pluronic

Overview Organs-on-chips have proved to be extremely valuable systems utilized for disease modeling, drug testing, and mimicking organ function. This organ-on-a-chip protocol allows you to build structures with controlled inner

Bioprinting Collagen for Layered Tissues

Overview Collagen type I is the most abundant protein in the extracellular matrix and has been widely used in academia and industry for tissue engineering applications. With Allevi’s exclusive CORE™

Bioprinting Liver Tissue

Overview Liver tissue function is determined by the interaction of cells with their microenvironment, namely through the interpretation of biochemical and mechanical signals present in its tissue-specific extracellular matrix. Hence,