The Allevi Blog

Bioprinting Vascularized Soft Tissue

Overview Tissue vasculature is essential to maintaining its viability and functionality, however, there are currently no simple ways to create thick vascularized tissue. This protocol enables researchers to create thick

Conductive Tissue Bioprinting Protocol

Overview 3D-Graphene (3DG) is a biocompatible, conductive material utilized for printing electrically stimulated tissues such as cardiac, skeletal muscle and nerve, as well as biocompatible circuits. 3DG is printed at

Fibroblast Bioprinting Protocol

Overview Skin engineering is a prominent alternative to traditional methods used for wound healing, drug testing & discovery, and other skin-related applications. However, challenges remain when it comes to generating

Bioprinting Bone Tissue Using Hyperelastic Bone

Overview Hyperelastic Bone™ is a material made by Dimension Inx composed of over 90% hydroxyapatite, a naturally occurring mineral from bone tissue. Hyperelastic Bone™ is printed at room temperature and

Bioprinting GelMA

Overview GelMA (gelatin methacrylate) is a gelatin-based hydrogel that reacts and crosslinks in the presence of LAP and Allevi blue light technology (1,2). The recommended preparation provided in the user

General Aseptic Techniques

If in need of a refresher, review this aseptic technique video. Remember to turn on your biosafety cabinet at least 15 minutes before use. Wipe it down with kim-wipes and

Changing Cell Media (General)

Overview This protocol will walk you through the process of changing cell media, also known as feeding cells. Please note that different cell types need to be fed at different

Passaging Cells (General)

Overview Passaging is required when cells have proliferated to confluences around 80%. This important step enables you to culture enough cells for your experiments, as well as maintain the health

Thawing Cells (General)

Overview Thawing cells properly is crucial to cell culture health. Usually, specific cell lines will have specific thawing protocols provided by the manufacturer. This is a general protocol for thawing

Freezing Cells (General)

Overview Freezing cells is a useful step to prolong the use of a cell lot. This is a general protocol for freezing cells that do not accompany a manufacturer protocol.

How to Bioprint a Buckyball Using Fresh

Below are print files and instructions that will walk you through how to bioprint a buckyball using the FRESH method. Please note: Print settings are for a 2% sodium alginate

Bioprinting Experiment Preparation in 8 Easy Steps

After you’ve used our printing parameters guide for your biomaterial of choice or you’ve completed the biomaterial optimization yourself, you are ready to start bioprinting with cells! Before you start,