Overview Tissue vasculature is essential to maintaining its viability and functionality, however, there are currently no simple ways to create thick vascularized tissue. This protocol enables researchers to create thick
Overview 3D-Graphene (3DG) is a biocompatible, conductive material utilized for printing electrically stimulated tissues such as cardiac, skeletal muscle and nerve, as well as biocompatible circuits. 3DG is printed at
Overview Skin engineering is a prominent alternative to traditional methods used for wound healing, drug testing & discovery, and other skin-related applications. However, challenges remain when it comes to generating
Overview Hyperelastic Bone™ is a material made by Dimension Inx composed of over 90% hydroxyapatite, a naturally occurring mineral from bone tissue. Hyperelastic Bone™ is printed at room temperature and
If in need of a refresher, review this aseptic technique video. Remember to turn on your biosafety cabinet at least 15 minutes before use. Wipe it down with kim-wipes and
Overview This protocol will walk you through the process of changing cell media, also known as feeding cells. Please note that different cell types need to be fed at different
Overview Passaging is required when cells have proliferated to confluences around 80%. This important step enables you to culture enough cells for your experiments, as well as maintain the health
Overview Thawing cells properly is crucial to cell culture health. Usually, specific cell lines will have specific thawing protocols provided by the manufacturer. This is a general protocol for thawing
Overview Freezing cells is a useful step to prolong the use of a cell lot. This is a general protocol for freezing cells that do not accompany a manufacturer protocol.
Below are print files and instructions that will walk you through how to bioprint a buckyball using the FRESH method. Please note: Print settings are for a 2% sodium alginate
After you’ve used our printing parameters guide for your biomaterial of choice or you’ve completed the biomaterial optimization yourself, you are ready to start bioprinting with cells! Before you start,
After preparing for a biostudy, it’s time to mix them with the biomaterial of your choice and load your cell-laden bioink into the syringe. Below we present a general guide
