Many primary and stem cell cultures have unpredictable proliferation rates, making it difficult to plan studies. While there will always be a degree of unpredictability, tracking and analyzing experimental data
Introduction Poly(lactic-co-glycolic acid), or PLGA, is a common thermoplastic used in bioprinting for its tunable degradable and mechanical properties[1,2]. The degradation and mechanical properties of this material can be altered
Percoll is a well-referenced media for density gradient centrifugation of cells, viruses and subcellular particles(1). Percoll is composed of colloidal silica coated with polyvinylpyrrolidone (PVP). Percoll has also been previously
Case study: Bioprinting Hydroxyapatite and PCL — Hydroxyapatite and polycaprolactone blends have been previously investigated as scaffolds for bone tissue engineering [1]. In addition to its biocompatibility, hydroxyapatite offers osteoconductive
This study tested the effects of various steps in the polymerization process on cell viability with photoinitiators Irgacure and LAP are mixed with GelMA. Introduction Photopolymerization is a common process
Introduction Poly(ethyelene glycol) diacrylate, or PEGDA, is one of the most common synthetic hydrogels used for cell encapsulation. The advantages of this biomaterial include biocompatibility, hydrophilicity and the ability to
Introduction Gelatin methacrylate, a common photopolymerizable biomaterial, is a gelatin-based hydrogel modified with a methacrylate group that crosslinks through free radical polymerization. Derived from collagen, gelatin methacrylate exhibits many native
Introduction LifeInk 200 is a high concentration collagen bioink useful for bioprinting structures with high shape fidelity. As the most common protein in many tissues’ ECMs, collagen-based bioinks are great
Introduction As a naturally occurring polysaccharide commonly derived from algae or seaweed, sodium alginate’s abundance and low cost make it a popular biomaterial for cellular encapsulation and bioprinting [1]. Sodium
Freeform reversible embedding of suspended hydrogels, also known as the FRESH method, allows for the bioprinting of soft cell encapsulated materials such as collagen or alginate (1). If you’re unfamiliar
Whether you have a novel material that you need to characterize or you want to further optimize a more common material, it’s critical to ensure that your print parameters result
Welcome to the Allevi quick start guide to bioprinting parameters for every bioink! The following bioprinting parameters are general guidelines for bioinks offered on the Allevi platform, but with some
