The Allevi Blog

How To Use A Cell Culture Tracker

Many primary and stem cell cultures have unpredictable proliferation rates, making it difficult to plan studies. While there will always be a degree of unpredictability, tracking and analyzing experimental data

Viability Report: Bioprinted PLGA

Introduction Poly(lactic-co-glycolic acid), or PLGA, is a common thermoplastic used in bioprinting for its tunable degradable and mechanical properties[1,2]. The degradation and mechanical properties of this material can be altered

Percoll Improves Bioprinted Cell Distribution

Percoll is a well-referenced media for density gradient centrifugation of cells, viruses and subcellular particles(1). Percoll is composed of colloidal silica coated with polyvinylpyrrolidone (PVP). Percoll has also been previously

Case Study: Bioprinting Hydroxyapatite and PCL

Case study: Bioprinting Hydroxyapatite and PCL — Hydroxyapatite and polycaprolactone blends have been previously investigated as scaffolds for bone tissue engineering [1]. In addition to its biocompatibility, hydroxyapatite offers osteoconductive

Viability Report: LAP and Irgacure with GelMA

This study tested the effects of various steps in the polymerization process on cell viability with photoinitiators Irgacure and LAP are mixed with GelMA. Introduction Photopolymerization is a common process

Viability Report: Bioprinted PEGDA

Introduction Poly(ethyelene glycol) diacrylate, or PEGDA, is one of the most common synthetic hydrogels used for cell encapsulation. The advantages of this biomaterial include biocompatibility, hydrophilicity and the ability to

Viability Report: Bioprinted GelMA and LAP

Introduction Gelatin methacrylate, a common photopolymerizable biomaterial, is a gelatin-based hydrogel modified with a methacrylate group that crosslinks through free radical polymerization. Derived from collagen, gelatin methacrylate exhibits many native

Viability Report: Bioprinted Collagen

Introduction LifeInk 200 is a high concentration collagen bioink useful for bioprinting structures with high shape fidelity. As the most common protein in many tissues’ ECMs, collagen-based bioinks are great

Viability Report: Bioprinted Alginate

Introduction As a naturally occurring polysaccharide commonly derived from algae or seaweed, sodium alginate’s abundance and low cost make it a popular biomaterial for cellular encapsulation and bioprinting [1]. Sodium

The FRESH Method

Freeform reversible embedding of suspended hydrogels, also known as the FRESH method, allows for the bioprinting of soft cell encapsulated materials such as collagen or alginate (1). If you’re unfamiliar

Print Parameter Optimization Guide

Whether you have a novel material that you need to characterize or you want to further optimize a more common material, it’s critical to ensure that your print parameters result

Bioprinting Parameters for Every Bioink Cheat Sheet

Welcome to the Allevi quick start guide to bioprinting parameters for every bioink! The following bioprinting parameters are general guidelines for bioinks offered on the Allevi platform, but with some